Biochemistry Class Notebook

Biochem Lab Fall 2009

2009-08-31T10:59:00.000-07:00

BIOCHEM LAB: Spectrophotometry of Riboflavin Part I

2009-08-26T05:35:00.000-07:00

(click the title to play or right click it to download and play in Quicktime)

The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part I before coming to class.

If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.

BIOCHEM LAB: Spectrophotometry of Riboflavin Part II

2009-08-25T23:14:00.000-07:00

(click the title to play or right click to download and play in Quicktime)

The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part II video before class.

If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.

BIOCHEM LAB: pH Determination

2009-08-24T15:09:00.000-07:00

Read your manual and do your calculations before class. Let me know if you have trouble viewing the Quicktime movie.

BIOCHEM LAB: Titration of an Amino Acid

2009-08-23T20:44:00.000-07:00

The amino acid titration experiment is described in this 20 minute video. CLICK the title to download and play a windows media file (not very good). Here you can download and play the quicktime file or get it from iTunes. Finally to accommodate all of you you may be able to view this video below. Go through it before taking the bonus quiz.

Protein Concentration Determination

2009-08-22T04:47:00.000-07:00

In the Experiment IV video will take a stock solution of BSA to generate a standard curve to use to determine the concentration of an unknown concentration of BSA. BSA is a standard size protein and is used regularly as a control to compare and determine concentrations of many different types of pure proteins. Can you tell me what BSA stands for?

Protein Characterization and PAG Electrophoresis

2009-08-21T09:49:00.000-07:00

Experiment VI and part of Experiment VII are described in this video. There is forthcoming the actual loading, running, staining and destaining of the gel before the final so stay tuned. You can access this video from iTunes or click the title here and view it or download it from my archives if having trouble viewing here. It may be necessary to wait until the video completes buffering to get a smooth view.

Determination of Catalase Activity

2009-08-20T21:51:00.000-07:00

Experiment VIII utilizes one of the fastest enzymes in biochemistry called catalase and its common household substrate none as hydrogen peroxide. Peroxide generates free oxygen radicals that can be damaging to tissues. Catalase along with other free radical scavengers and neutralizers converts peroxide into water and oxygen very efficiently. What factors effect the efficiency of catalase activity?

You can click the title here and view the video in my archive, you can download from iTunes of view here. You will have a better viewing experience if you wait for the video to buffer.

Alkaline Phosphatase Experiment #9

2009-08-19T14:27:00.000-07:00

Get it from Blackboard if unable to view here.

Blackboard Academic Suite

Introduction to Biochemistry

2009-07-30T19:30:00.000-07:00

I welcome your comments here after or before your class. Suggestions for topics and clarification may be and are encouraged to be posted here. You can be anonymous or you may identify yourself. I especially would like to see comment strings, that is, what you think about what someone else has said. Come here often to review material covered in lecture. Although I am not teaching lecture this semester you may still inquire of me with questions and comments. I am available to discuss these with you and will always support your instructor's efforts.

Chapter 1 part 1

2009-07-28T19:20:00.000-07:00

Much of chapter 1 is a review of material you should have seen before. This takes you into an introduction to the chemical and physical properties of water...Chapter 2.

Thermodynamics....huh?

2009-07-27T05:42:00.000-07:00

So here's a little description of what I believe explains the difference between
&Delta G and &Delta G⁰.

Gibb's Free Energy

Chapter 1 part 2

2009-07-02T19:10:00.000-07:00

Chapter 1 contains a discussion of thermodynamics. Your comments are always welcomed. Enthalpy, entropy and free energy changes lie underneath all biochemistry reactions driving them to completion and initiating the next reaction. Carefully read your chapter and search for other sources to reinforce these concepts.

pH and Amino Acid Titration

2009-06-20T04:20:00.000-07:00

Here is a quick review of pH, pK and pI that will be important when we begin Chapter 4: amino acids.Amino Acid Titration.

For Window Media Players

Chapter 4: Amino Acids

2009-06-18T23:00:00.000-07:00

Here is a summary of some important details concerning amino acid structure and properties. You should also be able to download this for free from the iTunes music store if you have an account. You will find it in the Podcast section under "Biochemistry Class Notebook".

Stevie...what more can I say.

Chapter 7

2009-06-17T06:09:00.000-07:00

After discussing amino acids, protein primary, secondary and tertiary structure, we discuss protein function using hemoglobin: the classical allosteric protein.

The Bohr Effect

2009-06-16T05:11:00.000-07:00

Here's a brief overview of the most challenging topic in chapter 7, The Bohr Effect.

Michaelis-Menton Kinetics after Exam II

2009-03-25T06:44:00.000-07:00

Here is the first of 2 installments on our Enzyme Kinetics lectures. Use this for a quick review of the terms K_M, V_MAX, K_cat, K_S and enzyme efficiency. More description of the Linweaver-Burke and inhibition will follow in the 2nd installment.

Good luck Ming!

Enzyme Inhibition

2009-03-24T20:28:00.000-07:00

Hope you listen in on this description of the 3 common enzyme inhibition profiles, it may prove helpful on the Exam III.

DNA Replication part I

2009-03-21T05:53:00.000-07:00

In my absence begin to review eukaryotic DNA replication in Chapter 24 using the following notes as your guide. There are 3 parts that cover the first half of this chapter on DNA replication. A second set will follow to cover DNA repair mechanisms

Part I

DNA Replication part II

2009-03-20T17:53:00.000-07:00

Chapter 24 DNA Replication part III

2009-03-01T10:55:00.000-08:00

Summer time...and the livin' is easy...

2008-08-30T11:27:00.000-07:00

That's right. All done for another summer of kicking back, relaxin', readin', writing and some overall reflectin'. Tenure decision approaching in December so will be getting my show together to present to the jury. Regardless of the outcome I've been bless to be a part of this and look forward to continuing my Biochemistry Notebook wherever I might be.

Welcome back!

2008-08-22T13:00:00.000-07:00

Summer's over so let's get back to work. I hope to provide you with some insight into your biochemistry experiments here as well as recycle the notes on lecture topics. To start off the semester lets have a party...a Chemistry Party. (be warned, some reaction may be disturbing in the following video!)

We Need Your Data!!

2008-03-31T07:45:00.000-07:00

Are you a graduating senior or alumni from Xavier in Chemistry? We need you to fill in the survey. (click the title to redirect you)